RAF1 (pS338) Antibody

Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.

Target	RAF1 (pS338)
Clonality	Polyclonal
Target Modification	Ser338
Modification	Phosphorylation
Reactivity	Human
Tested Applications	ELISA, WB, DB
Host	Rabbit
Recommended dilutions	WB: 1/1000, DB: 1/500. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S338 of human RAF1.
Isotype	IgG
Form	Liquid
Purification	Purified by protein A affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with peptides. The antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P04049 (UniProt, ExPASy)
UniProt Secondary AC	B0LPH8, B2R5N3, Q15278, Q9UC20
UniProt Entry Name	RAF1_HUMAN
NCBI Accession	NP_002871.1
KEGG	hsa:5894
String	9606.ENSP00000251849
Molecular Weight	Calculated MW: 73.1 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Mouse, Rat, Cow and Chicken RAF1.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on RAF1 (pS338)

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